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The anion exchange resin, DEAE, is shown here NH and you have three proteins each with a different isoelectric point (pl):
The anion exchange resin, DEAE, is shown here NH and you have three proteins each with a different isoelectric point (pl):
Protein A pl = 7.0
Protein B pl = 5.0
Protein C pl = 9.0
At which pH would you need to make a buffer to allow only protein B to bind?
pH 3.0
pH 6.0
pH 8.0
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